Journal: Nature Communications

Article Title: Lymph nodes molecular subtypes unravel lymph nodes heterogeneity and clinical implications in colorectal cancer

doi: 10.1038/s41467-025-63200-z

Figure Lengend Snippet: Dot plot of PCA based on fibroblasts-specific ( A ) and endothelial cell-specific ( B ) gene markers to estimate their ability in distinguish the LNs subtypes ( n = 68). C Box plots illustrating the differences in COL1A1, α-SMA, FN1, CD31, and LYVE1 gene expression between LNs subtypes ( n = 50, 35, 31, 32, 11, and 14). Each data point represents an individual LN (biological replicates). Box plots represent the median (center line), 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). The mIF staining and quantification reveal differential expression of α-SMA, FN1, COL1A1 ( n = 20 and 16) ( D ) and LYVE-1, CD31 ( n = 24 and 18) ( E ) between NLN_C1 and NLN_C4. Each data point represents an individual LN (biological replicates). Data are presented as mean values ± SD. The p value was calculated using a two-sided Student’s t test. Scale bars indicate 100 μm, 500 μm, and 1000 μm respectively. Source data are provided as a Source Data file. F Sirius Red staining results of differences in fibrosis levels between NLN_C1 and NLN_C4. Scale bars indicate 1000 μm. PC principal component, PCA principal component analysis, mIF multiplex immunofluorescence, SD standard deviation.

Article Snippet: The primary antibodies included rabbit polyclonal antibody CXCL13/BCA1 (Proteintech, 10927-1-AP); rabbit polyclonal antibody LYVE1 (Proteintech, 28321-1-AP); mouse monoclonal antibody CD20 (Proteintech, 60271-1-Ig); mouse monoclonal antibody FN1 (Proteintech, 66042-1-Ig); mouse monoclonal antibody CD31 (Proteintech, 66065-2-Ig); mouse monoclonal antibody COL1A1 (Proteintech, 67288-1-Ig); mouse monoclonal antibody PDPN (Proteintech, 67432-1-Ig); mouse monoclonal antibody CD35 (Proteintech, 68033-1-Ig); rabbit recombinant antibody α-SMA (Proteintech, 80008-1-RR); All images were captured using an EVOS FL Auto 2 Imaging System (Thermo Fisher Scientific).

Techniques: Gene Expression, Staining, Quantitative Proteomics, Multiplex Assay, Immunofluorescence, Standard Deviation

Journal: Acta Histochemica et Cytochemica

Article Title: Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp

doi: 10.1267/ahc.24-00009

Figure Lengend Snippet: Localization of CD31 (+) and endomucin (+) vessels. ( A ) Stage of tooth crown formation at 1 week. Endomucin (+) vessels (red) mainly exist in the dental pulp. ( B ) Higher magnifications of the boxed regions in A . Both CD31(+) and endomucin (+) vessels (yellow) are narrow and located beneath the coronal dentin. ( C ) Higher magnifications of the boxed regions in B . Merged immunoreaction (yellow) is localized at thin vessels in the odontoblast layer. ( D ) Dental pulp at 4 weeks. Both CD31 (+) and endomucin (+) vessels decreased in the crown and increased in the root of dental pulp at 4 weeks. ( E ) Higher magnifications of the boxed regions in D . Thin vessels are colored in yellow, and express both CD31 (+) and endomucin (+) and located near the radicular dentin (arrowheads). ( F ) Higher magnifications of the boxed regions in E . Note the merged immunoreaction (yellow) shows mesh-like pattern. ( G ) Dental pulp at 12 weeks. Root canals at 12 week are narrower than those at 4 week. ( H ) Higher magnifications of the boxed regions in G . CD31 (+)-immunoreaction is mainly seen, and Both CD31 (+) and endomucin (+) vessels are faintly seen in dental pulp (arrowhead). ( I ) Higher magnifications of the boxed regions in H . CD31 (+) vessels are seen beneath the odontoblast layer. ( J ) Dental pulp at 56 weeks. Few CD31 (+) and endomucin (+) vessels were detected in dental pulp. ( K ) Higher magnifications of the boxed regions in J . Main vessels are positive for CD31 in the coronal pulp. ( L ) Higher magnifications of the boxed regions in K . Immuno-reaction for endomucin was separately seen at the CD31(+) vessels. Green: CD31 (+), Red: Endomucin (+), Yellow: CD31 (+) and endomucin (+). Arrowheads: CD31 (+) and endomucin (+) vessels. Den: dentin; Ob: odontoblast layer. Bars = 200 μm ( A , D , G , J ), 50 μm ( B , E , H , K ) and 15 μm ( C , F , I , L ).

Article Snippet: Sections of decalcified samples were incubated with an Alexa Fluor 488-conjugated anti-CD31/PECAM-1 goat polyclonal antibody (dilution 1:100, FAB3628G-10, R&D Systems, Minneapolis, MN, USA) and a rat monoclonal antibody against mouse endomucin (dilution 1:200, sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA) at 20 ± 5°C for 8 hr or 4°C overnight in the dark.

Techniques:

Journal: Acta Histochemica et Cytochemica

Article Title: Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp

doi: 10.1267/ahc.24-00009

Figure Lengend Snippet: Ratio of CD31 (+) and endomucin (+) vessels. Data represent means ± standard deviations (SD). Four mice were used for statistical analysis in each group. These ratios 12 and 56 weeks were significantly lower than those 1 and 4 weeks. * Statistical significance ( P < 0.05)

Article Snippet: Sections of decalcified samples were incubated with an Alexa Fluor 488-conjugated anti-CD31/PECAM-1 goat polyclonal antibody (dilution 1:100, FAB3628G-10, R&D Systems, Minneapolis, MN, USA) and a rat monoclonal antibody against mouse endomucin (dilution 1:200, sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA) at 20 ± 5°C for 8 hr or 4°C overnight in the dark.

Techniques:

Journal: Acta Histochemica et Cytochemica

Article Title: Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp

doi: 10.1267/ahc.24-00009

Figure Lengend Snippet: Calcein labeling and endomucin (+) vessels. ( A ) At 4 weeks after birth. Dentin with a large distance between the two calcein-lines was observed in the crown and root dentin. ( B ) Higher magnification of the boxed regions in A . Both CD31(+) and endomucin (+) vessels colored in white were thin and present directly beneath calcein-labeled dentin at all sites. ( C ) At 8 weeks after birth. Although the lines were present in root dentin, they were negligible in coronal dentin. The distance between the two lines at 8 weeks were narrower than that at 4 weeks. Both CD31 and endomucin (+) vessels were scattered in coronal dental pulp. ( D ) Higher magnification of the boxed regions in C . Both CD31 and endomucin (+) vessels were located at the odontoblast layer in root pulp. ( E ) At 12 weeks after birth. Calcein-labeled areas were only present in newly formed apical cementum, and were faint or not seen at coronal and root dentin. ( F ) Higher magnification of the boxed regions in E . CD31(+) and ndomucin (+) vessels were seen neat the newly formed cementum and faintly detected in dental pulp. Den: dentin, Cem: cementum, Ob: odontoblast layer, Pu: dental pulp. Bars = 200 μm ( A , C , E ), 30 μm ( B , D , F ). Green: calcein labeling, Cyan: CD31(+) vessel, Red: endomucin (+) vessel, White: both CD31(+) and endomucin(+) vessel, Blue: nuclear.

Article Snippet: Sections of decalcified samples were incubated with an Alexa Fluor 488-conjugated anti-CD31/PECAM-1 goat polyclonal antibody (dilution 1:100, FAB3628G-10, R&D Systems, Minneapolis, MN, USA) and a rat monoclonal antibody against mouse endomucin (dilution 1:200, sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA) at 20 ± 5°C for 8 hr or 4°C overnight in the dark.

Techniques: Labeling